This study provides a direct in vivo visualization of Ste2p tetramers and the pheromone effect on the extent of the receptor oligomerization. There are 100+ professionals named 'Martin Frster', who use LinkedIn to exchange information, ideas, and opportunities. Intriguingly, receptor tetramers in their active form showed a significant increase in FRET. View the profiles of professionals named 'Martin Frster' on LinkedIn. Our results suggest that in its quiescent (nonligand-activated) state, Ste2p is found at least as a tetrameric complex on the plasma membrane. The bimolecular fluorescence complementation (BiFC) assay was combined with FRET to further investigate the tetrameric complexes of Ste2p. Foster Wheeler Energy Corporation : SeeFujimoto, Yoshiji : SeeSteiner, Peter. Stimulation of Ste2p oligomers with the receptor ligand did not result in any significant change on observed FRET values. See, Declaration of Michael Martin on Behalf of the California Independent System. Foerster, Otto and Vojta, Erich, to Siemens Aktiengesellschaft. I Want to Live International Supervideos Itar-Tass Russian News Agency v. The Förster resonance energy transfer (FRET) technique was used to detect receptors’ oligomerization by calculating the energy transfer from EGFP to mCherry. Wikipedia:Date formatting and linking poll/List of articles with year-only links (3) Guinn v. In our study, Ste2p, a yeast mating pheromone GPCR, was tagged with enhanced green fluorescent protein (EGFP), mCherry, and with split florescent protein fragments at the receptor C-terminus. However, the nature and extent of their oligomerization is still under debate. Oligomerization of G protein-coupled receptors (GPCRs) may play important roles in maturation, internalization, signaling, and pharmacology of these receptors.